Civit, Laia and Moradzadeh, Nima and Jonczyk, Anna and Neckermann, Patrick and Asbach, Benedikt and Peterhoff, David and Wagner, Ralf and Famulok, Michael and Mayer, Guenter and Kjems, Jorgen and Valero, Julian (2024) A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25 (9): 4642. ISSN 1661-6596, 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The COVID-19 pandemic has underscored the critical need for the advancement of diagnostic and therapeutic platforms. These platforms rely on the rapid development of molecular binders that should facilitate surveillance and swift intervention against viral infections. In this study, we have evaluated by three independent research groups the binding characteristics of various published RNA and DNA aptamers targeting the spike protein of the SARS-CoV-2 virus. For this comparative analysis, we have employed different techniques such as biolayer interferometry (BLI), enzyme-linked oligonucleotide assay (ELONA), and flow cytometry. Our data show discrepancies in the reported specificity and affinity among several of the published aptamers and underline the importance of standardized methods, the impact of biophysical techniques, and the controls used for aptamer characterization. We expect our results to contribute to the selection and application of suitable aptamers for the detection of SARS-CoV-2.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SELEX; MINIPROTEINS; DOMAIN; aptamers; SARS-CoV-2; spike protein; COVID-19; comparative analysis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jan 2026 07:50 |
| Last Modified: | 28 Jan 2026 07:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64679 |
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