Schuele, Simone and Hackenbroch, Carsten and Beer, Meinrad and Ostheim, Patrick and Hermann, Cornelius and Muhtadi, Razan and Stewart, Samantha and Port, Matthias and Scherthan, Harry and Abend, Michael (2024) Tin prefiltration in computed tomography does not significantly alter radiation-induced gene expression and DNA double-strand break formation. PLOS ONE, 19 (12): e0315808. ISSN 1932-6203
Full text not available from this repository. (Request a copy)Abstract
Background The tin (Sn) prefilter technique is a recently introduced dose-saving technique in computed tomography (CT). This study investigates whether there is an altered molecular biological response in blood cells using the tin prefiltering technique. Methods Blood from 6 donors was X-irradiated ex-vivo with 20 mGy full dose (FD) protocols (Sn 150 kV, 150 kV, and 120 kV) and a tin prefiltered 16.5 mGy low dose (LD) protocol on a CT scanner. Biological changes were determined by quantification of gamma H2AX DNA double-strand break (DSB) foci, and differential gene expression (DGE) relative to unexposed samples were examined for seven known radiation-induced genes (FDXR, DDB2, BAX, CDKN1A, AEN, EDA2R, APOBEC3H) and 667 microRNAs (miRNA). Results EDA2R and DDB2 gene expression (GE) increased 1.7-6-fold (p = 0.0004-0.02) and average DNA DSB foci value (0.31 +/- 0.02, p<0.0001) increased significantly relative to unexposed samples, but similarly for the applied radiation protocols. FDXR upregulation (2.2-fold) was significant for FD protocols (p = 0.01-0.02) relative to unexposed samples. miRNA GE changes were not significant (p = 0.15-1.00) and DGE were similar for the examined protocols (p = 0.10-1.00). An increased frequency of lower DGE values was seen in the Sn 150 kV LD protocol compared to the 120 kV FD and Sn 150 kV FD protocols (p = 0.001-0.008). Conclusions The current ex-vivo study indicates no changes regarding transcriptional and post-transcriptional DGE and DNA DSB induction when using the tin prefilter technique and even a significant tendency to lower radiation-induced DGE-changes due to the dose reduction of the tin prefilter with equal image quality compared to classical CT scan protocols was found.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PULMONARY NODULE DETECTION; ULTRALOW-DOSE CT; MICRORNA BIOGENESIS; IMAGE QUALITY; EX-VIVO; REDUCTION; RESPONSES; CELLS |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Röntgendiagnostik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jan 2026 06:31 |
| Last Modified: | 28 Jan 2026 06:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64715 |
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