Bommer, Thea and Knierim, Maria and Unsoeld, Julia and Riedl, Dominic and Stengel, Laura and Paulus, Michael and Koertl, Thomas and Liaw, Norman and Maier, Lars S. and Streckfuss-Boemeke, Katrin and Sossalla, Samuel and Pabel, Steffen (2024) Simulation of cardiac arrhythmias in human induced pluripotent stem cell-derived cardiomyocytes. PLOS ONE, 19 (9): e0310463. ISSN 1932-6203
Full text not available from this repository. (Request a copy)Abstract
The effects and mechanisms of cardiac arrhythmias are still incompletely understood and an important subject of cardiovascular research. A major difficulty for investigating arrhythmias is the lack of appropriate human models. Here, we present a protocol for a translational simulation of different types of arrhythmias using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and electric cell culture pacing. The protocol comprises the handling of ventricular and atrial hiPSC-CM before and during in vitro arrhythmia simulation and possible arrhythmia simulation protocols mimicking clinical arrhythmias like atrial fibrillation. Isolated or confluent hiPSC-CM can be used for the simulation. In vitro arrhythmia simulation did not impair cell viability of hiPSC-CM and could reproduce arrhythmia associated phenotypes of patients. The use of hiPSC-CM enables patient-specific studies of arrhythmias, genetic interventions, or drug-screening. Thus, the in vitro arrhythmia simulation protocol may offer a versatile tool for translational studies on the mechanisms and treatment options of cardiac arrhythmias.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEART-FAILURE; ATRIAL-FIBRILLATION; MODEL |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jan 2026 06:35 |
| Last Modified: | 28 Jan 2026 06:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64731 |
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