Timm, Thomas and Hild, Christiane and Liebisch, Gerhard and Rickert, Markus and Lochnit, Guenter and Steinmeyer, Juergen (2024) Functional Insights into the Sphingolipids C1P, S1P, and SPC in Human Fibroblast-like Synoviocytes by Proteomic Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25 (15): 8363. ISSN 1661-6596, 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The (patho)physiological function of the sphingolipids ceramide-1-phosphate (C1P), sphingosine-1-phosphate (S1P), and sphingosylphosphorylcholine (SPC) in articular joints during osteoarthritis (OA) is largely unknown. Therefore, we investigated the influence of these lipids on protein expression by fibroblast-like synoviocytes (FLSs) from OA knees. Cultured human FLSs (n = 7) were treated with 1 of 3 lipid species-C1P, S1P, or SPC-IL-1 beta, or with vehicle. The expression of individual proteins was determined by tandem mass tag peptide labeling followed by high-resolution electrospray ionization (ESI) mass spectrometry after liquid chromatographic separation (LC-MS/MS/MS). The mRNA levels of selected proteins were analyzed using RT-PCR. The 3sphingolipids were quantified in the SF of 18 OA patients using LC-MS/MS. A total of 4930 proteins were determined using multiplex MS, of which 136, 9, 1, and 0 were regulated both reproducibly and significantly by IL-1 beta, C1P, S1P, and SPC, respectively. In the presence of IL-1 ss, all 3 sphingolipids exerted ancillary effects. Only low SF levels of C1P and SPC were found. In conclusion, the 3 lipid species regulated proteins that have not been described in OA. Our results indicate that charged multivesicular body protein 1b, metal cation symporter ZIP14, glutamine-fructose-6-P transaminase, metallothionein-1F and -2A, ferritin, and prosaposin are particularly interesting proteins due to their potential to affect inflammatory, anabolic, catabolic, and apoptotic mechanisms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SPHINGOSINE 1-PHOSPHATE; CERAMIDE 1-PHOSPHATE; CELL-MIGRATION; GROWTH-FACTOR; SPHINGOSINE-1-PHOSPHATE; EXPRESSION; PLASMA; SPHINGOSYLPHOSPHORYLCHOLINE; BIOSYNTHESIS; INVOLVEMENT; ceramide-1-phosphate; sphingosine-1-phosphate; sphingosylphosphorylcholine; lysosphingomyelin; sphingolipid; proteomics; inflammation; mass spectrometry; functions; osteoarthritis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jan 2026 08:00 |
| Last Modified: | 28 Jan 2026 08:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64761 |
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