Esih, Hana and Mezgec, Klemen and Billmeier, Martina and Malensek, Spela and Bencina, Mojca and Grilc, Blaz and Vidmar, Sara and Gasperlin, Mirjana and Bele, Marjan and Zidarn, Mihaela and Zupanc, Tatjana Lejko and Morgan, Tina and Jordan, Ingo and Sandig, Volker and Schroedel, Silke and Thirion, Christian and Protzer, Ulrike and Wagner, Ralf and Lainscek, Dusko and Jerala, Roman (2024) Mucoadhesive film for oral delivery of vaccines for protection of the respiratory tract. JOURNAL OF CONTROLLED RELEASE, 371. pp. 179-192. ISSN 0168-3659, 1873-4995
Full text not available from this repository. (Request a copy)Abstract
The delivery of vaccines plays a pivotal role in influencing the strength and longevity of the immune response and controlling reactogenicity. Mucosal immunization, as compared to parenteral vaccination, could offer greater protection against respiratory infections while being less invasive. While oral vaccination has been presumed less effective and believed to target mainly the gastrointestinal tract, trans-buccal delivery using mucoadhesive films (MAF) may allow targeted delivery to the mucosa. Here we present an effective strategy for mucosal delivery of several vaccine platforms incorporated in MAF, including DNA plasmids, viral vectors, and lipid nanoparticles incorporating mRNA (mRNA/LNP). The mRNA/LNP vaccine formulation targeting SARSCoV-2 as a proof of concept remained stable within MAF consisting of slowly releasing water-soluble polymers and an impermeable backing layer, facilitating enhanced penetration into the oral mucosa. This formulation elicited antibody and cellular responses comparable to the intramuscular injection, but also induced the production of mucosal IgAs, highlighting its efficacy, particularly for use as a booster vaccine and the potential advantage for protection against respiratory infections. The MAF vaccine preparation demonstrates significant advantages, such as efficient delivery, stability, and simple noninvasive administration with the potential to alleviate vaccine hesitancy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | T-CELL; PENETRATION ENHANCERS; PERMEATION ENHANCERS; IMMUNE-RESPONSES; MESSENGER-RNA; VIRUS; IMMUNIZATION; REPLICATION; FORMULATION; STABILITY; Vaccine delivery; Mucoadhesive film; Mucosal immunity; Respiratory infection |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Jan 2026 09:45 |
| Last Modified: | 14 Jan 2026 09:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64767 |
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