Vesela, Barbora and Bzduskova, Jana and Ramesova, Alice and Svandova, Eva and Graessel, Susanne and Matalova, Eva (2024) Inhibition of caspase-11 under inflammatory conditions suppresses chondrogenic differentiation. TISSUE & CELL, 89: 102425. ISSN 0040-8166
Full text not available from this repository. (Request a copy)Abstract
Caspase-11 is the murine homologue of human caspases-4 and -5 and is involved in mediating the inflammatory response. However, its functions are often confused and misinterpreted with the more important and better described caspase-1. Therefore, this study focused exclusively on the specific roles of caspase-11, both in cartilage formation and in the inflammatory environment. The presence of caspase-11 during mouse limb development and in chondrogenic cell cultures was investigated by immunofluorescence detection. Subsequently, the function of caspase-11 was downregulated and the affected molecules investigated. The expression analysis applied for osteo/chondrogenesis associated factors and inflammatory cytokines. Simultaneously, morphological appearance of the micromass cultures was evaluated. The results revealed that caspase-11 is physiologically present during cartilage development, but its inhibition under physiological conditions has no significant effect on chondrogenic differentiation. However, in an inflammatory environment, inhibition and downregulation of caspase-11 leads to reduced differentiation of cartilage nodules. Additionally, reduced expression of several genes including Col2a1 and Sp7 and conversely increased expression of Mmp9 were observed. In the cytokine expression panel, a significant decrease was found in molecules that, along with the inflammatory function, may also be involved in cartilage differentiation. The findings bring new information about caspase-11 in chondrogenesis and show that its downregulation under inflammatory conditions reduces cartilage formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXPRESSION; PYROPTOSIS; ACTIVATION; PROMOTES; Caspase-11; Osteoarthritis; Inflammation; Cartilage; Chondrogenesis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jan 2026 08:52 |
| Last Modified: | 15 Jan 2026 08:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64830 |
Actions (login required)
 |
View Item |
