Gong, Wen and Bak, Deniz Tiambeng and Wendrich, Jos R. and Weijers, Dolf and Laux, Thomas (2024) CDC48A, an interactor of WOX2, is required for embryonic patterning in Arabidopsis thaliana. PLANT CELL REPORTS, 43 (7): 174. ISSN 0721-7714, 1432-203X
Full text not available from this repository. (Request a copy)Abstract
During Arabidopsis embryo patterning, the WUSCHEL HOMEOBOX 2 (WOX2) transcription factor is a major regulator of protoderm and shoot stem cell initiation. Loss of WOX2 function results in aberrant protodermal cell divisions and, redundantly with its paralogs WOX1, WOX3, and WOX5, compromised shoot meristem formation. To elucidate the molecular basis for WOX2 function, we searched for protein interactors by IP-MS/MS from WOX2-overexpression roots displaying reprogramming toward shoot-like cell fates. Here, we report that WOX2 directly interacts with the type II AAA ATPase molecular chaperone CELL DIVISION CYCLE 48A (CDC48A). We confirmed this interaction with bimolecular fluorescence complementation and co-immunoprecipitation and found that both proteins co-localize in the nucleus. We show that CDC48A loss of function results in protoderm and shoot meristem stem cell initiation defects similar to WOX2 loss of function. We also provide evidence that CDC48A promotes WOX2 activity independently of proteolysis or the regulation of nuclear localization, common mechanisms of CDC48A function in other processes. Our results point to a new role of CDC48A in potentiating WOX2 function during early embryo patterning.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STEM-CELL HOMEOSTASIS; AAA-ATPASE; SHOOT MERISTEM; WUSCHEL; EXPRESSION; PROTEIN; DIVISION; GENES; MOVEMENT; FATE; Embryogenesis; Shoot meristem; Arabidopsis thaliana; WOX2; CDC48A |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Nov 2025 09:39 |
| Last Modified: | 18 Nov 2025 09:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64837 |
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