Brunner, Sarah and Liebisch, Gerhard and Schweizer, Sabine and Scheiber, Josef and Giansanti, Piero and Hidrobo, Maria and Hermeling, Sven and Oeckl, Josef and Mello, Natalia Prudente de and Perocchi, Fabiana and Seeliger, Claudine and Strohmeyer, Akim and Klingenspor, Martin and Plagge, Johannes and Kuester, Bernhard and Burkhardt, Ralph and Ecker, Josef (2024) Mitochondrial lipidomes are tissue speci fi c - low cholesterol contents relate to UCP1 activity. LIFE SCIENCE ALLIANCE, 7 (8): E202402828. ISSN 2575-1077
Full text not available from this repository. (Request a copy)Abstract
Lipid composition is conserved within sub -cellular compartments to maintain cell function. Lipidomic analyses of liver, muscle, white and brown adipose tissue (BAT) mitochondria revealed substantial differences in their glycerophospholipid (GPL) and free cholesterol (FC) contents. The GPL to FC ratio was 50 -fold higher in brown than white adipose tissue mitochondria. Their purity was veri fied by comparison of proteomes with ER and mitochondria -associated membranes. A lipid signature containing PC and FC, calculated from the lipidomic pro files, allowed differentiation of mitochondria from BAT of mice housed at different temperatures. Elevating FC in BAT mitochondria prevented uncoupling protein (UCP) 1 function, whereas increasing GPL boosted it. Similarly, STARD3 overexpression facilitating mitochondrial FC import inhibited UCP1 function in primary brown adipocytes, whereas a knockdown promoted it. We conclude that the mitochondrial GPL/FC ratio is key for BAT function and propose that targeting it might be a promising strategy to promote UCP1 activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BROWN ADIPOSE-TISSUE; TANDEM MASS-SPECTROMETRY; FATTY-ACID SYNTHESIS; PHYSIOLOGICAL REGULATION; UNCOUPLING PROTEIN; FLIP-FLOP; TRANSPORT; PHOSPHOLIPIDS; MECHANISM; CELLS |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jan 2026 11:29 |
| Last Modified: | 22 Jan 2026 11:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64901 |
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