Bartsch, Sibylle and Kohnert, Eva and Kreutz, Clemens and Woelber, Johan P. and Anderson, Annette and Burkhardt, Ann-Sophie and Hellwig, Elmar and Buchalla, Wolfgang and Hiller, Karl-Anton and Ratka-Krueger, Petra and Cieplik, Fabian and Al-Ahmad, Ali (2024) Chlorhexidine digluconate mouthwash alters the oral microbial composition and affects the prevalence of antimicrobial resistance genes. FRONTIERS IN MICROBIOLOGY, 15: 1429692. ISSN 1664-302X
Full text not available from this repository. (Request a copy)Abstract
Introduction Chlorhexidine (CHX) is a commonly used antiseptic in situations of limited oral hygiene ability such as after periodontal surgery. However, CHX is also considered as a possible factor in the emergence of cross-resistance to antibiotics. The aim of this study was to analyze the changes in the oral microbiota and the prevalence of antimicrobial resistance genes (ARGs) due to CHX treatment.Materials and methods We analyzed the oral metagenome of 20 patients who applied a 0.2% CHX mouthwash twice daily for 4 weeks following periodontal surgical procedures. Saliva and supragingival plaque samples were examined before, directly after 4 weeks, and another 4 weeks after discontinuing the CHX treatment.Results Alpha-diversity decreased significantly with CHX use. The Bray-Curtis dissimilarity increased in both sample sites and mainly streptococci showed a higher relative abundance after CHX treatment. Although no significant changes of ARGs could be detected, an increase in prevalence was found for genes that encode for tetracycline efflux pumps.Conclusion CHX treatment appears to promote a caries-associated bacterial community and the emergence of tetracycline resistance genes. Future research should focus on CHX-related changes in the microbial community and whether the discovered tetracycline resistance genes promote resistance to CHX.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ANTIBIOTIC-RESISTANCE; STREPTOCOCCUS-PNEUMONIAE; MACROLIDE-RESISTANCE; ANTISEPTICS; ADAPTATION; BIOCIDES; DISTINCT; PROTEUS; EFFLUX; HEALTH; chlorhexidine; cross-resistance; antibiotics; metagenome; streptococci; caries; efflux pump; tetracycline |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jan 2026 07:35 |
| Last Modified: | 28 Jan 2026 07:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64918 |
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