Sklenarova, Dorota and Hlavacek, Antonin and Krivankova, Jana and Brandmeier, Julian C. and Weisova, Julie and Rihacek, Michal and Gorris, Hans H. and Skladal, Petr and Farka, Zdenek (2024) Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles. LAB ON A CHIP, 24 (14). pp. 3536-3545. ISSN 1473-0197, 1473-0189
Full text not available from this repository. (Request a copy)Abstract
Early-stage diagnosis of prostatic carcinoma is essential for successful treatment and, thus, significant prognosis improvement. In laboratory practice, the standard non-invasive diagnostic approach is the immunochemical detection of the associated biomarker, prostate-specific antigen (PSA). Ultrasensitive detection of PSA is essential for both diagnostic and recurrence monitoring purposes. To achieve exceptional sensitivity, we have developed a microfluidic device with a flow-through cell for single-molecule analysis using photon-upconversion nanoparticles (UCNPs) as a detection label. For this purpose, magnetic microparticles (MBs) were first optimized for the capture and preconcentration of PSA and then used to implement a bead-based upconversion-linked immunoassay (ULISA) in the microfluidic device. The digital readout based on counting single nanoparticle-labeled PSA molecules on MBs enabled a detection limit of 1.04 pg mL-1 (36 fM) in 50% fetal bovine serum, which is an 11-fold improvement over the respective analog MB-based ULISA. The microfluidic technique conferred several other advantages, such as easy implementation and the potential for achieving high-throughput analysis. Finally, it was proven that the microfluidic setup is suitable for clinical sample analysis, showing a good correlation with a reference electrochemiluminescence assay (recovery rates between 97% and 105%). A microfluidic device for single-molecule detection of prostate-specific antigen was developed, utilizing magnetic microparticles as a solid phase and photon-upconversion nanoparticles as a label.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMMUNOASSAY; FUTURE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Nov 2025 10:47 |
| Last Modified: | 11 Nov 2025 10:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65005 |
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