Huang, Shan and He, Hailong and Tom, Robby Zachariah and Glasl, Sarah and Anzenhofer, Pia and Stiel, Andre C. and Hofmann, Susanna M. and Ntziachristos, Vasilis (2024) Non-invasive optoacoustic imaging of dermal microcirculatory revascularization in diet-induced obese mice undergoing exercise intervention. PHOTOACOUSTICS, 38: 100628. ISSN 2213-5979
Full text not available from this repository. (Request a copy)Abstract
Microcirculatory dysfunction has been observed in the dermal white adipose tissue (dWAT) and subcutaneous white adipose tissue (scWAT) of obese humans and has been proposed as an early prediction marker for cardiometabolic disease progression. In-vivo visualization and longitudinal monitoring of microvascular remodeling in these tissues remains challenging. We compare the performance of two optoacoustic imaging methods, i.e. multispectral optoacoustic tomography (MSOT) and raster-scanning optoacoustic mesoscopy (RSOM) in visualizing lipid and hemoglobin contrast in scWAT and dWAT in a mouse model of diet-induced obesity (DIO) undergoing voluntary wheel running intervention for 32 weeks. MSOT visualized lipid and hemoglobin contrast in murine fat depots in a quantitative manner even at early stages of DIO. We show for the first time to our knowledge that RSOM allows precise visualization of the dWAT microvasculature and provides quantitative readouts of skin layer thickness and vascular density in dWAT and dermis. Combination of MSOT and RSOM resolved exerciseinduced morphological changes in microvasculature density, tissue oxygen saturation, lipid and blood volume content in dWAT and scWAT. The combination of MSOT and RSOM may allow precise monitoring of microcirculatory dysfunction and intervention response in dWAT and scWAT in a mouse model for DIO. Our findings have laid out the foundation for future clinical studies using optoacoustic-derived vascular readouts from adipose tissues as a biomarker for monitoring microcirculatory function in metabolic disease.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Obesity; Cardio-metabolic disease; Non-invasive optoacoustic imaging; Dermal microvascular function; Exercise-induced revascularization |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Dec 2025 09:40 |
| Last Modified: | 09 Dec 2025 09:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65065 |
Actions (login required)
 |
View Item |
