Liang, Chunguang and Spoerl, Silvia and Xiao, Yin and Habenicht, Katharina M. and Haeusl, Sigrun S. and Sandner, Isabel and Winkler, Julia and Strieder, Nicholas and Eder, Ruediger and Stanewsky, Hanna and Alexiou, Christoph and Dudziak, Diana and Rosenwald, Andreas and Edinger, Matthias and Rehli, Michael and Hoffmann, Petra and Winkler, Thomas H. and Berberich-Siebelt, Friederike (2024) Oligoclonal CD4⁺CXCR5⁺ T cells with a cytotoxic phenotype appear in tonsils and blood. COMMUNICATIONS BIOLOGY, 7 (1): 879. ISSN 2399-3642
Full text not available from this repository. (Request a copy)Abstract
In clinical situations, peripheral blood accessible CD3(+)CD4(+)CXCR5(+) T-follicular helper (T-FH) cells may have to serve as a surrogate indicator for dysregulated germinal center responses in tissues. To determine the heterogeneity of T-FH cells in peripheral blood versus tonsils, CD3(+)CD4(+)CD45RA(-)CXCR5(+) cells of both origins were sorted. Transcriptomes, TCR repertoires and cell-surface protein expression were analysed by single-cell RNA sequencing, flow cytometry and immunohistochemistry. Reassuringly, all blood-circulating CD3(+)CD4(+)CXCR5(+) T-cell subpopulations also appear in tonsils, there with some supplementary T-FH characteristics, while peripheral blood-derived T-FH cells display markers of proliferation and migration. Three further subsets of T-FH cells, however, with bona fide T-follicular gene expression patterns, are exclusively found in tonsils. One additional, distinct and oligoclonal CD4(+)CXCR5(+) subpopulation presents pronounced cytotoxic properties. Those 'killer T-FH (T-FK) cells' can be discovered in peripheral blood as well as among tonsillar cells but are located predominantly outside of germinal centers. They appear terminally differentiated and can be distinguished from all other T-FH subsets by expression of NKG7 (TIA-1), granzymes, perforin, CCL5, CCR5, EOMES, CRTAM and CX3CR1. All in all, this study provides data for detailed CD4(+)CXCR5(+) T-cell assessment of clinically available blood samples and extrapolation possibilities to their tonsil counterparts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FOLLICULAR HELPER-CELLS; CXC CHEMOKINE RECEPTOR-5; MEMORY TFH CELLS; REGULATORY CELLS; B-CELLS; ANTIGEN; RESPONSES; BCL-6; LYMPHOCYTES; PROMOTE |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2025 06:20 |
| Last Modified: | 02 Dec 2025 06:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65104 |
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