Osadare, Ibukun Elizabeth and Monecke, Stefan and Abdilahi, Abdinasir and Mueller, Elke and Collatz, Maximilian and Braun, Sascha and Reissig, Annett and Schneider-Brachert, Wulf and Kieninger, Baerbel and Eichner, Anja and Rath, Anca and Fritsch, Juergen and Gary, Dominik and Frankenfeld, Katrin and Wellhoefer, Thomas and Ehricht, Ralf (2024) Fast and Economic Microarray-Based Detection of Species-, Resistance-, and Virulence-Associated Genes in Clinical Strains of Vancomycin-Resistant Enterococci (VRE). SENSORS, 24 (19): 6476. ISSN 1424-8220
Full text not available from this repository.Abstract
Today, there is a continuous worldwide battle against antimicrobial resistance (AMR) and that includes vancomycin-resistant enterococci (VRE). Methods that can adequately and quickly detect transmission chains in outbreaks are needed to trace and manage this problem fast and cost-effectively. In this study, DNA-microarray-based technology was developed for this purpose. It commenced with the bioinformatic design of specific oligonucleotide sequences to obtain amplification primers and hybridization probes. Microarrays were manufactured using these synthesized oligonucleotides. A highly parallel and stringent labeling and hybridization protocol was developed and employed using isolated genomic DNA from previously sequenced (referenced) clinical VRE strains for optimal sensitivity and specificity. Microarray results showed the detection of virulence, resistance, and species-specific genes in the VRE strains. Theoretical predictions of the microarray results were also derived from the sequences of the same VRE strain and were compared to array results while optimizing protocols until the microarray result and theoretical predictions were a match. The study concludes that DNA microarray technology can be used to quickly, accurately, and economically detect specifically and massively parallel target genes in enterococci.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SEQUENCE TYPING SCHEME; ANTIBIOTIC-RESISTANCE; EXPRESSION; FAECALIS; CLONES; microarrays; enterococci; VRE; DNA; resistance genes; virulence genes; Gram-positive bacteria |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Krankenhaushygiene und Infektiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Dec 2025 06:49 |
| Last Modified: | 16 Dec 2025 06:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65123 |
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