Dirks, Johannes and Fischer, Jonas and Klaussner, Julia and Hofmann, Christine and Holl-Wieden, Annette and Buck, Viktoria and Klemann, Christian and Girschick, Hermann J. and Caruana, Ignazio and Erhard, Florian and Morbach, Henner (2024) Disease-specific T cell receptors maintain pathogenic T helper cell responses in postinfectious Lyme arthritis. JOURNAL OF CLINICAL INVESTIGATION, 134 (17): e179391. ISSN 0021-9738, 1558-8238
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND. Antibiotic-Refractory Lyme Arthritis (ARLA) involves a complex interplay of T cell responses targeting Borrelia burgdorferi antigens progressing toward autoantigens by epitope spreading. However, the precise molecular mechanisms driving the pathogenic T cell response in ARLA remain unclear. Our aim was to elucidate the molecular program of diseasespecific Th cells. METHODS. Using flow cytometry, high-throughput T cell receptor (TCR) sequencing, and scRNA-Seq of CD4+ + Th cells isolated from the joints of patients with ARLA living in Europe, we aimed to infer antigen specificity through unbiased analysis of TCR repertoire patterns, identifying surrogate markers for disease-specific TCRs, and connecting TCR specificity to transcriptional patterns. RESULTS. PD-1hiHLA-DR+CD4+ hi HLA-DR + CD4 + effector T cells were clonally expanded within the inflamed joints and persisted throughout disease course. Among these cells, we identified a distinct TCR-J3 motif restricted to HLA-DRB1*11 or *13 alleles. These alleles, being underrepresented in patients with ARLA living in North America, were unexpectedly prevalent in our European cohort. The identified TCR-J3 motif served as surrogate marker for a convergent TCR response specific to ARLA, distinguishing it from other rheumatic diseases. In the scRNA-Seq data set, the TCR-J3 motif particularly mapped to peripheral T helper (TPH) PH ) cells displaying signs of sustained proliferation, continuous TCR signaling, and expressing CXCL13 and IFN-gamma. CONCLUSION. By inferring disease-specific TCRs from synovial T cells we identified a convergent TCR response in the joints of patients with ARLA that continuously fueled the expansion of T PH cells expressing a pathogenic cytokine effector program. The identified TCRs will aid in uncovering the major antigen targets of the maladaptive immune response. FUNDING. Supported by the German Research Foundation (DFG) MO 2160/4-1; the Federal Ministry of Education and Research (BMBF; Advanced Clinician Scientist-Program INTERACT; 01EO2108) embedded in the Interdisciplinary Center for Clinical Research (IZKF) of the University Hospital W & uuml;rzburg; the German Center for Infection Research (DZIF; Clinical Leave Program; TI07.001_007) and the Interdisciplinary Center for Clinical Research (IZKF) W & uuml;rzburg (Clinician Scientist Program, Z-2/CSP-30).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACE PROTEIN-A; HLA-DR MOLECULES; BORRELIA-BURGDORFERI; TREATMENT-RESISTANT; SYNOVIAL-FLUID; GROWTH-FACTOR; ASSOCIATION; DOMINANT; TARGET; RECOGNITION; |
| Subjects: | 000 Computer science, information & general works > 004 Computer science |
| Divisions: | Informatics and Data Science > Department Computational Life Science > Chair of Computational Immunology (Prof. Dr. Florian Erhard) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jan 2026 14:33 |
| Last Modified: | 15 Jan 2026 14:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65182 |
Actions (login required)
 |
View Item |
