Krenek, Tomas and Pola, Josef and Stich, Theresia and Pattappa, Girish and Docheva, Denitsa and Kostejn, Martin and Medlin, Rostislav and Mikysek, Petr and Jandova, Vera and Pola, Michal and Kovarik, Tomas and Cassel, Julia and Holzman, Martin and Moskal, Denys (2024) Hydrolytic instability of laser-ablatively deposited CaSi2 coatings in air and neutral water affects the behavior of bone healing-related cell types. SURFACES AND INTERFACES, 55: 105381. ISSN 2468-0230
Full text not available from this repository. (Request a copy)Abstract
Calcium silicide (CaSi2) instability in humid air or pH-neutral water remains unknown, although the topochemical formation of silicene from CaSi2 in various aqueous phases is a well-known process. Here we report on laser ablation of CaSi2 in the vacuum and ethanol, characterize coatings deposited on titanium substrates with electron microscopy, X-ray photoelectron, Raman, and infrared spectroscopy, and examine the instability of the deposited coatings in humid air and neutral water. We further investigate the behavior of human mesenchymal stromal cells (hMSCs), vascular cells (HUVECS, human umbilical vein endothelial cells), and macrophages (derived from THP-1 cell line) in contact with the deposited coatings submerged in cell culture medium. The observed results indicate that the CaSi2 coatings undergo topochemical conversion to silicene, which is accompanied by hydrolytic reactions leading to inorganic Ca compounds and SiO2, and that the response of all cell types in the hydrolyzed CaSi2 surface domain is negatively affected by the nature of the hydrolytic products. In contrast, cells showed a tendency for enhanced biocompatibility towards the CaSi2 particles ablated in the vacuum. The results suggest that coating approaches can significantly influence cell behavior outcomes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Calcium silicide; Laser ablation in a vacuum; Laser ablation in liquid; CaSi 2 hydrolysis; Bioactivity; Mesenchymal stromal cells; HUVECs; Macrophages; biocompatibility |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Oct 2025 06:08 |
| Last Modified: | 30 Oct 2025 06:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65377 |
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