Merignac-Lacombe, Jeanne and Kornbausch, Nicole and Sivarajan, Rinu and Boichot, Valentin and Berg, Kevin and Oberwinkler, Heike and Saliba, Antoine-Emmanuel and Loos, Helene M. and Kasemo, Totta Ehret and Scherzad, Agmal and Bodem, Jochen and Buettner, Andrea and Neiers, Fabrice and Erhard, Florian and Hackenberg, Stephan and Heydel, Jean-Marie and Steinke, Maria (2024) Characterization of a Human Respiratory Mucosa Model to Study Odorant Metabolism. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 72 (22). pp. 12696-12706. ISSN 0021-8561, 1520-5118
Full text not available from this repository. (Request a copy)Abstract
Nasal xenobiotic metabolizing enzymes (XMEs) are important for the sense of smell because they influence odorant availability and quality. Since the major part of the human nasal cavity is lined by a respiratory mucosa, we hypothesized that this tissue contributed to nasal odorant metabolism through XME activity. Thus, we built human respiratory tissue models and characterized the XME profiles using single-cell RNA sequencing. We focused on the XMEs dicarbonyl and l-xylulose reductase, aldehyde dehydrogenase (ALDH) 1A1, and ALDH3A1, which play a role in food odorant metabolism. We demonstrated protein abundance and localization in the tissue models and showed the metabolic activity of the corresponding enzyme families by exposing the models to the odorants 3,4-hexandione and benzaldehyde. Using gas chromatography coupled with mass spectrometry, we observed, for example, a significantly higher formation of the corresponding metabolites 4-hydroxy-3-hexanone (39.03 +/- 1.5%, p = 0.0022), benzyl alcohol (10.05 +/- 0.88%, p = 0.0008), and benzoic acid (8.49 +/- 0.57%, p = 0.0004) in odorant-treated tissue models compared to untreated controls (0 +/- 0, 0.12 +/- 0.12, and 0.18 +/- 0.18%, respectively). This is the first study that reveals the XME profile of tissue-engineered human respiratory mucosa models and demonstrates their suitability to study nasal odorant metabolism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DICARBONYL/L-XYLULOSE REDUCTASE; MECHANISM; KETOCONAZOLE; INHIBITION; EXPRESSION; NOSE; nasal odorant metabolism; human respiratory tissue models; xenobiotic metabolizing enzymes; perireceptor events; tissue engineering |
| Subjects: | 300 Social sciences > 360 Social services; association 600 Technology > 640 Home economics & family living |
| Divisions: | Informatics and Data Science > Department Computational Life Science > Chair of Computational Immunology (Prof. Dr. Florian Erhard) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Dec 2025 06:35 |
| Last Modified: | 04 Dec 2025 06:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65383 |
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