Min, Kyung-Won and Choi, Kyoung-Min and Mun, Hyejin and Ko, Seungbeom and Lee, Ji Won and Sagum, Cari A. and Bedford, Mark T. and Kim, Young-Kook and Delaney, Joe R. and Cho, Jung-Hyun and Dawson, Ted M. and Dawson, Valina L. and Twal, Waleed and Kim, Dong-Chan and Panganiban, Clarisse H. and Lang, Hainan and Zhou, Xin and Shin, Seula and Hu, Jian and Heise, Tilman and Kwon, Sang-Ho and Kim, Dongsan and Kim, Young Hwa and Kang, Sung-Ung and Kim, Kyungmin and Lewis, Sydney and Eroglu, Ahmet and Ryu, Seonghyun and Kim, Dongin and Chang, Jeong Ho and Jung, Junyang and Yoon, Je-Hyun (2024) Mature microRNA-binding protein QKI suppresses extracellular microRNA let-7b release. JOURNAL OF CELL SCIENCE, 137 (21): jcs261575. ISSN 0021-9533, 1477-9137
Full text not available from this repository. (Request a copy)Abstract
Argonaute (AGO), a component of RNA-induced silencing complexes (RISCs), is a representative RNA-binding protein (RBP) known to bind with mature microRNAs (miRNAs) and is directly involved in post-transcriptional gene silencing. However, despite the biological significance of miRNAs, the roles of other miRNA-binding proteins (miRBPs) remain unclear in the regulation of miRNA loading, dissociation from RISCs and extracellular release. In this study, we performed protein arrays to profile miRBPs and identify 118 RBPs that directly bind to miRNAs. Among those proteins, the RBP quaking (QKI) inhibits extracellular release of the mature microRNA let-7b by controlling the loading of let-7b into extracellular vesicles via additional miRBPs such as AUF1 (also known as hnRNPD) and hnRNPK. The enhanced extracellular release of let-7b after QKI depletion activates Toll-like receptor 7 (TLR7) and promotes the production of proinflammatory cytokines in recipient cells, leading to brain inflammation in the mouse cortex. Thus, this study reveals the contribution of QKI to the inhibition of brain inflammation via regulation of extracellular let-7b release.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESSENGER-RNA; CIRCULATING MICRORNAS; GENE; CANCER; EXPRESSION; COMPENDIUM; VESICLES; MIRNAS; MIR-21; QKI; AGO2; RNA-binding protein; let-7b; Extracellular vesicular miRNA |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Pädiatrische Hämatologie, Onkologie und Stammzelltransplantation |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Aug 2025 06:49 |
| Last Modified: | 14 Aug 2025 06:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65647 |
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