Acott, Ted S. and Fautsch, Michael P. and Mao, Weiming and Ethier, C. Ross and Huang, Alex S. and Kelley, Mary J. and Aga, Mini and Bhattacharya, Sanjoy K. and Borras, Terete and Bovenkamp, Diane and Chowdhury, Uttio Roy and Clark, Abbot F. and Dibas, Mohammed I. and Du, Yiqin and Elliott, Michael H. and Faralli, Jennifer A. and Gong, Haiyan and Herberg, Samuel and Johnstone, Murray A. and Kaufman, Paul L. and Keller, Kate E. and Kelly, Ruth A. and Krizaj, David and Kuehn, Markus H. and Li, Hoi Lam and Lieberman, Raquel and Lin, Shan C. and Liu, Yutao and Mcdonnell, Fiona S. and Mcdowell, Colleen M. and Mclellan, Gillian J. and Mzyk, Philip and Nair, Kayarat Saidas and Overby, Darryl R. and Peters, Donna M. and Raghunathan, Vijaykrishna and Rao, Ponugoti Vasantha and Roddy, Gavin W. and Sharif, Najam A. and Shim, Myoung Sup and Sun, Yang and Thomson, Benjamin R. and Toris, Carol B. and Willoughby, Colin E. and Zhang, Hao F. and Freddo, Thomas F. and Fuchshofer, Rudolf and Hill, Kamisha R. and Karimi, Alireza and Kizhatil, Krishnakumar and Kopcyznski, Casey C. and Liton, Paloma and Patel, Gaurang and Peng, Michael and Pattabiraman, Padmanabhan P. and Prasanna, Ganesh and Reina-Torres, Ester and Samples, E. Griffen and Samples, John R. and Steel, Cynthia L. and Strohmaier, Clemens A. and Subramanian, Preeti and Sugali, Chenna Kesavulu and van Batenburg-Sherwood, Joseph and Wong, Cydney and Youngblood, Hannah and Zode, Gulab S. and White, Elizabeth and Stamer, W. Daniel (2024) Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 65 (14): 32. ISSN 0146-0404, 1552-5783
Full text not available from this repository. (Request a copy)Abstract
Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AQUEOUS-HUMOR OUTFLOW; HUMAN TRABECULAR MESHWORK; OPEN-ANGLE GLAUCOMA; ORGAN-CULTURE; HUMAN EYES; OCULAR HYPERTENSION; ANTERIOR SEGMENTS; CATIONIC FERRITIN; CELLS; FLOW; intraocular pressure regulation models; perfused anterior segment organ culture; ex vivo aqueous humor outflow regulation models |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jul 2025 10:15 |
| Last Modified: | 23 Jul 2025 10:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65744 |
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