Torta, Federico and Hoffmann, Nils and Burla, Bo and Alecu, Irina and Arita, Makoto and Bamba, Takeshi and Bennett, Steffany A. L. and Bertrand-Michel, Justine and Bruegger, Britta and Cala, Monica P. and Camacho-Munoz, Dolores and Checa, Antonio and Chen, Michael and Chocholouskova, Michaela and Cinel, Michelle and Chu-Van, Emeline and Colsch, Benoit and Coman, Cristina and Connell, Lisa and Sousa, Bebiana C. and Dickens, Alex M. and Fedorova, Maria and Eiriksson, Finnur Freyr and Gallart-Ayala, Hector and Ghorasaini, Mohan and Giera, Martin and Guan, Xue Li and Haid, Mark and Hankemeier, Thomas and Harms, Amy and Hoering, Marcus and Holcapek, Michal and Hornemann, Thorsten and Hu, Chunxiu and Hulsmeier, Andreas J. and Huynh, Kevin and Jones, Christina M. and Ivanisevic, Julijana and Izumi, Yoshihiro and Koefeler, Harald C. and Lam, Sin Man and Lange, Mike and Lee, Jong Cheol and Liebisch, Gerhard and Lippa, Katrice and Lopez-Clavijo, Andrea F. and Manzi, Malena and Martinefski, Manuela R. and Math, Raviswamy G. H. and Mayor, Satyajit and Meikle, Peter J. and Monge, Maria Eugenia and Moon, Myeong Hee and Muralidharan, Sneha and Nicolaou, Anna and Nguyen-Tran, Thao and O'Donnell, Valerie B. and Oresic, Matej and Ramanathan, Arvind and Riols, Fabien and Saigusa, Daisuke and Schock, Tracey B. and Schwartz-Zimmermann, Heidi and Shui, Guanghou and Singh, Madhulika and Takahashi, Masatomo and Thorsteinsdottir, Margret and Tomiyasu, Noriyuki and Tournadre, Anthony and Tsugawa, Hiroshi and Tyrrell, Victoria J. and van der Gugten, Grace and Wakelam, Michael O. and Wheelock, Craig E. and Wolrab, Denise and Xu, Guowang and Xu, Tianrun and Bowden, John A. and Ekroos, Kim and Ahrends, Robert and Wenk, Markus R. (2024) Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards. NATURE COMMUNICATIONS, 15 (1): 8562. ISSN , 2041-1723
Full text not available from this repository. (Request a copy)Abstract
In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs <= 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LIQUID-CHROMATOGRAPHY; LIPIDOMICS; MS; QUANTIFICATION; LDL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jul 2025 09:08 |
| Last Modified: | 23 Jul 2025 09:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65761 |
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