Batschkus, Sarah and Atanassov, Ilian and Lenz, Christof and Meyer-Marcotty, Philipp and Cingoez, Gokhan and Kirschneck, Christian and Urlaub, Henning and Miosge, Nicolai (2017) Mapping the secretome of human chondrogenic progenitor cells with mass spectrometry. ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 212. pp. 4-10. ISSN 0940-9602, 1618-0402
Full text not available from this repository. (Request a copy)Abstract
Tissue engineering offers promising perspectives in the therapy of osteoarthritis. In the context of cell based therapy, chondrogenic progenitor cells (CPCs) may be used to regenerate defects in cartilage tissue. An in-depth characterization of the secretome of CPCs is a prerequisite to this approach. In this study, a method was developed for the qualitative and quantitative analysis of the secretome of undifferentiated and differentiated CPCs. Secreted proteins from cells grown in two-dimensional as well as three-dimensional alginate cultures were extracted and analyzed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Quantitation was achieved by internal standardization using stable isotope-labeled amino acids in cell culture (SILAC). Qualitative analysis of CPC secretomes revealed ECM components, signal proteins and growth factors most of which were also found in healthy cartilage. A quantitative comparison revealed significantly upregulated proteins with regenerative potential during differentiation, while proteins involved in catabolic metabolism were significantly downregulated. The development of methods for qualitative and quantitative analysis of the secretome of CPCs by mass spectrometry provides a foundation for the investigation of progenitor or stem cells from other sources. (C) 2017 Elsevier GmbH. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARTICULAR CHONDROCYTES; QUANTITATIVE PROTEOMICS; OSTEOARTHRITIS; QUANTIFICATION; MECHANISMS; EXPRESSION; PROTEIN-1; COMPLEXES; CULTURE; TISSUE; Osteoarthritis; Secretome; Chondrogenic progenitor cells; Mass spectrometry; Tissue engineering |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 13 Feb 2019 14:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/661 |
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