Loughran, Gary and Andreev, Dmitry E. and Terenin, Ilya M. and Namy, Olivier and Mikl, Martin and Yordanova, Martina M. and Mcmanus, C. Joel and Firth, Andrew E. and Atkins, John F. and Fraser, Christopher S. and Ignatova, Zoya and Iwasaki, Shintaro and Kufel, Joanna and Larsson, Ola and Leidel, Sebastian A. and Mankin, Alexander S. and Mariotti, Marco and Tanenbaum, Marvin E. and Topisirovic, Ivan and Vazquez-Laslop, Nora and Viero, Gabriela and Caliskan, Neva and Chen, Yiwen and Clark, Patricia L. and Dinman, Jonathan D. and Farabaugh, Philip J. and Gilbert, Wendy V. and Ivanov, Pavel and Kieft, Jeffrey S. and Muhlemann, Oliver and Sachs, Matthew S. and Shatsky, Ivan N. and Sonenberg, Nahum and Steckelberg, Anna-Lena and Willis, Anne E. and Woodside, Michael T. and Valasek, Leos Shivaya and Dmitriev, Sergey E. and Baranov, Pavel V. (2025) Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR). NATURE STRUCTURAL & MOLECULAR BIOLOGY, 32 (3). pp. 418-430. ISSN 1545-9993, 1545-9985
Full text not available from this repository. (Request a copy)Abstract
Dual reporters encoding two distinct proteins within the same mRNA have had a crucial role in identifying and characterizing unconventional mechanisms of eukaryotic translation. These mechanisms include initiation via internal ribosomal entry sites (IRESs), ribosomal frameshifting, stop codon readthrough and reinitiation. This design enables the expression of one reporter to be influenced by the specific mechanism under investigation, while the other reporter serves as an internal control. However, challenges arise when intervening test sequences are placed between these two reporters. Such sequences can inadvertently impact the expression or function of either reporter, independent of translation-related changes, potentially biasing the results. These effects may occur due to cryptic regulatory elements inducing or affecting transcription initiation, splicing, polyadenylation and antisense transcription as well as unpredictable effects of the translated test sequences on the stability and activity of the reporters. Unfortunately, these unintended effects may lead to misinterpretation of data and the publication of incorrect conclusions in the scientific literature. To address this issue and to assist the scientific community in accurately interpreting dual-reporter experiments, we have developed comprehensive guidelines. These guidelines cover experimental design, interpretation and the minimal requirements for reporting results. They are designed to aid researchers conducting these experiments as well as reviewers, editors and other investigators who seek to evaluate published data.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERNAL RIBOSOME ENTRY; CAP-INDEPENDENT TRANSLATION; MESSENGER-RNA TRANSLATION; INITIATING TRANSLATION; DEPENDENT TRANSLATION; BROAD-SPECTRUM; READ-THROUGH; 5' UTR; REINITIATION; EFFICIENCY; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Mar 2026 06:08 |
| Last Modified: | 24 Mar 2026 06:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/68084 |
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