Zander, Adrian and Willkomm, Sarah and Ofer, Sapir and van Wolferen, Marleen and Egert, Luisa and Buchmeier, Sabine and Stoeckl, Sarah and Tinnefeld, Philip and Schneider, Sabine and Klingl, Andreas and Albers, Sonja-Verena and Werner, Finn and Grohmann, Dina (2017) Guide-independent DNA cleavage by archaeal Argonaute from Methanocaldococcus jannaschii. NATURE MICROBIOLOGY, 2 (6): 17034. ISSN 2058-5276,
Full text not available from this repository. (Request a copy)Abstract
Prokaryotic Argonaute proteins acquire guide strands derived from invading or mobile genetic elements, via an unknown pathway, to direct guide-dependent cleavage of foreign DNA. Here, we report that Argonaute from the archaeal organism Methanocaldococcus jannaschii (MjAgo) possesses two modes of action: the canonical guide-dependent endonuclease activity and a non-guided DNA endonuclease activity. The latter allows MjAgo to process long double-stranded DNAs, including circular plasmid DNAs and genomic DNAs. Degradation of substrates in a guide-independent fashion primes MjAgo for subsequent rounds of DNA cleavage. Chromatinized genomic DNA is resistant to MjAgo degradation, and recombinant histones protect DNA from cleavage in vitro. Mutational analysis shows that key residues important for guide-dependent target processing are also involved in guide-independent MjAgo function. This is the first characterization of guide-independent cleavage activity for an Argonaute protein potentially serving as a guide biogenesis pathway in a prokaryotic system.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STRUCTURAL BASIS; BACTERIAL ARGONAUTE; SILENCING COMPLEX; RNA INTERFERENCE; RECOGNITION; SULFOLOBUS; PROTEINS; GENUS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Dina Grohmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:10 |
| Last Modified: | 25 Feb 2019 11:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/739 |
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