Mayr, Judith and Grijalvo, Santiago and Bachl, Juergen and Pons, Ramon and Eritja, Ramon and Diaz Diaz, David (2017) Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18 (6): 1139. ISSN 1422-0067,
Full text not available from this repository. (Request a copy)Abstract
Three different ionene polymers with varying quaternary ammonium moieties were used as a proof of concept for the formulation of antisense oligonucleotides, which are capable of inhibiting Renilla luciferase messenger ribonucleic acid (mRNA). Cationic vesicles, consisting of cationic polymer, antisense oligonucleotide (Luc) and non-ionic surfactant polysorbate 80, were investigated regarding their potential, cytotoxicity and transfection efficiency. Deoxyribonucleic acid- (DNA) forming complexes in the presence of cationic vesicles were also investigated in terms of small-angle X-ray scattering (SAXS). The studied cationic vesicles showed very little, if any, toxicity against HeLa cells. Transfection abilities proved to vary strongly depending on the present quaternary ammonium moiety.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENE DELIVERY; DNA; POLYMERS; TOXICITY; AMPHIPHILE; EFFICIENCY; HYDRATION; IONENES; COMPLEX; AGENTS; antisense oligonucleotide; cationic polymers; cationic vesicles; ionenes; transfection; polycations; polyplexes; quaternary ammonium |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Prof. Dr. David Díaz Díaz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:10 |
| Last Modified: | 28 Feb 2019 10:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/752 |
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