Resentini, Francesca and Cyprys, Philipp and Steffen, Joshua G. and Alter, Svenja and Morandini, Piero and Mizzotti, Chiara and Lloyd, Alan and Drews, Gary N. and Dresselhaus, Thomas and Colombo, Lucia and Sprunck, Stefanie and Masiero, Simona (2017) SUPPRESSOR OF FRIGIDA (SUF4) Supports Gamete Fusion via Regulating Arabidopsis EC1 Gene Expression. PLANT PHYSIOLOGY, 173 (1). pp. 155-166. ISSN 0032-0889, 1532-2548
Full text not available from this repository. (Request a copy)Abstract
The EGG CELL1 (EC1) gene family of Arabidopsis (Arabidopsis thaliana) comprises five members that are specifically expressed in the egg cell and redundantly control gamete fusion during double fertilization. We investigated the activity of all five EC1 promoters in promoter-deletion studies and identified SUF4 (SUPPRESSOR OF FRIGIDA4), a C2H2 transcription factor, as a direct regulator of the EC1 gene expression. In particular, we demonstrated that SUF4 binds to all five Arabidopsis EC1 promoters, thus regulating their expression. The down-regulation of SUF4 in homozygous suf4-1 ovules results in reduced EC1 expression and delayed sperm fusion, which can be rescued by expressing SUF4-b-glucuronidase under the control of the SUF4 promoter. To identify more gene products able to regulate EC1 expression together with SUF4, we performed coexpression studies that led to the identification of MOM1 (MORPHEUS' MOLECULE1), a component of a silencing mechanism that is independent of DNA methylation marks. In mom1-3 ovules, both SUF4 and EC1 genes are down-regulated, and EC1 genes show higher levels of histone 3 lysine-9 acetylation, suggesting that MOM1 contributes to the regulation of SUF4 and EC1 gene expression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FEMALE GAMETOPHYTE DEVELOPMENT; EGG-CELL; TRANSCRIPTION FACTORS; FINGER PROTEIN; EMBRYO SAC; WILD-TYPE; FERTILIZATION; THALIANA; COMPLEX; FATE; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 12:58 |
| Last Modified: | 22 Feb 2019 10:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/90 |
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