Wang, Xiao-Hui and Peng, Hong-Shang and Yang, Lin and You, Fang-Tian and Teng, Feng and Hou, Ling-Ling and Wolfbeis, Otto S. (2014) Targetable Phosphorescent Oxygen Nanosensors for the Assessment of Tumor Mitochondrial Dysfunction By Monitoring the Respiratory Activity. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 53 (46). pp. 12471-12475. ISSN 1433-7851, 1521-3773
Full text not available from this repository. (Request a copy)Abstract
Cellular respiration is a worthwhile criterion to evaluate mitochondrial dysfunction by measuring the dissolved oxygen. However, most of the existing sensing strategies merely report extracellular (ec-) or intracellular (ic-) O-2 rather than intramitochondrial (im-) O-2. Herein we present a method to assess tumor mitochondrial dysfunction with three phosphorescent nanosensors, which respond to ec-, ic-, and im-O-2. Time-resolved luminescence is applied to determine the respective oxygen consumption rates (OCRs) under varying respiratory conditions. Data obtained for the OCRs and on (intra)cellular O-2 gradients demonstrate that mitochondria in tumor cells are distinctly less active than those of healthy cells, resulting from restrained glucose utilization of and physical injury to the mitochondria. We believe that such a site-resolved sensing strategy can be applied to numerous other situations, for example to evaluate the adverse effects of drug candidates.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SENSITIVE PROBES; CELLS; TRIPHENYLPHOSPHONIUM; NANOPARTICLES; FLUORESCENCE; SENSORS; CANCER; luminescence; mitochondrial dysfunction; nanoparticles; oxygen; sensors |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Aug 2019 13:37 |
| Last Modified: | 08 Aug 2019 13:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9212 |
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