Penzkofer, Alfons and Stierl, Manuela and Mathes, Tilo and Hegemann, Peter (2014) Absorption and emission spectroscopic characterization of photo-dynamics of photoactivated adenylyl cyclase mutant bPAC-Y7F of Beggiatoa sp. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 140. pp. 182-193. ISSN 1011-1344,
Full text not available from this repository. (Request a copy)Abstract
The photoactivated cyclase bPAC of the microbial mats bacterium Beggiatoa sp. consists of a BLUF domain and an adenylyl cyclase domain. It has strong activity of photo-induced cyclic adenylyl monophosphate (cAMP) formation and is therefore an important optogenetic tool in neuroscience applications. The SUMO-bPAC-Y7F mutant where Tyr-7 is replaced by Phe-7 in the BLUF domain has lost the typical BLUF domain photo-cycle dynamics. Instead, the investigated SUMO-bPAC-Y7F mutant consisted of three protein conformations with different triplet based photo-dynamics: (i) reversible flavin quinone (Fl) cofactor reduction to flavin semiquinone (FlH), (ii) reversible violet/near ultraviolet absorbing flavin photoproduct (FlA) formation, and (iii) irreversible red absorbing flavin photoproduct (FlC) formation. Absorption and emission spectroscopic measurements on SUMO-bPAC-Y7F were carried out before, during and after light exposure. Flavin photo-dynamics schemes are developed for the SUMO-bPAC-Y7F fractions performing photo-induced FlH., FIA, and FlC formation. Quantitative parameters of the flavin cofactor excitation, relaxation and recovery dynamics in SUMO-bPAC-Y7F are determined. (C) 2014 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEG-M STRAIN; ELECTRON-TRANSFER; CHLAMYDOMONAS-REINHARDTII; AQUEOUS-SOLUTION; FLAVIN MONONUCLEOTIDE; RHODOBACTER-SPHAEROIDES; FLUORESCENCE DYNAMICS; CHARGE-TRANSFER; QUANTUM YIELD; BLUF PROTEIN; Photoactivated cyclase bPAC; BLUF domain; Soil bacterium Beggiatoa sp.; Triplet based flavin semiquinone photo-cycle; Flavin-C4a-adduct photo-cycle; Anionic flavin hydoquinone charge transfer complex formation |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Aug 2019 08:27 |
| Last Modified: | 08 Aug 2019 08:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9242 |
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