The Iho670 Fibers of Ignicoccus hospitalis Are Anchored in the Cell by a Spherical Structure Located beneath the Inner Membrane

Meyer, Carolin and Heimerl, Thomas and Wirth, Reinhard and Klingl, Andreas and Rachel, Reinhard (2014) The Iho670 Fibers of Ignicoccus hospitalis Are Anchored in the Cell by a Spherical Structure Located beneath the Inner Membrane. JOURNAL OF BACTERIOLOGY, 196 (21). pp. 3807-3815. ISSN 0021-9193, 1098-5530

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Abstract

The Iho670 fibers of the hyperthermophilic crenarchaeon of Ignicoccus hospitalis were shown to contain several features that indicate them as type IV pilus-like structures. The application of different visualization methods, including electron tomography and the reconstruction of a three-dimensional model, enabled a detailed description of a hitherto undescribed anchoring structure of the cell appendages. It could be identified as a spherical structure beneath the inner membrane. Furthermore, pools of the fiber protein Iho670 could be localized in the inner as well as the outer cellular membrane of I. hospitalis cells and in the tubes/vesicles in the intermembrane compartment by immunological methods.

Item Type: Article
Uncontrolled Keywords: OUTER-MEMBRANE; ARCHAEAL FLAGELLUM; IV PILI; HALOBACTERIUM-SALINARIUM; ELECTRON-MICROSCOPY; BACTERIAL FLAGELLA; MOTILITY STRUCTURE; PROTEINS; ULTRASTRUCTURE; VISUALIZATION;
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Reinhard Rachel
Depositing User: Dr. Gernot Deinzer
Date Deposited: 08 Aug 2019 10:47
Last Modified: 08 Aug 2019 10:47
URI: https://pred.uni-regensburg.de/id/eprint/9301

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