Pereira, Ana Marta and Masiero, Simona and Nobre, Margarida Sofia and Costa, Mario Luis and Solis, Maria-Teresa and Testillano, Pilar S. and Sprunck, Stefanie and Coimbra, Slvia (2014) Differential expression patterns of arabinogalactan proteins in Arabidopsis thaliana reproductive tissues. JOURNAL OF EXPERIMENTAL BOTANY, 65 (18). pp. 5459-5471. ISSN 0022-0957, 1460-2431
Full text not available from this repository. (Request a copy)Abstract
Arabinogalactan proteins (AGPs) are heavily glycosylated proteins existing in all members of the plant kingdom and are differentially distributed through distinctive developmental stages. Here, we showed the individual distributions of specific Arabidopsis AGPs: AGP1, AGP9, AGP12, AGP15, and AGP23, throughout reproductive tissues and indicated their possible roles in several reproductive processes. AGP genes specifically expressed in female tissues were identified using available microarray data. This selection was confirmed by promoter analysis using multiple green fluorescent protein fusions to a nuclear localization signal, beta-glucuronidase fusions, and in situ hybridization as approaches to confirm the expression patterns of the AGPs. Promoter analysis allowed the detection of a specific and differential presence of these proteins along the pathway followed by the pollen tube during its journey to reach the egg and the central cell inside the embryo sac. AGP1 was expressed in the stigma, style, transmitting tract, and the chalazal and funiculus tissues of the ovules. AGP9 was present along the vasculature of the reproductive tissues and AGP12 was expressed in the stigmatic cells, chalazal and funiculus cells of the ovules, and in the septum. AGP15 was expressed in all pistil tissues, except in the transmitting tract, while AGP23 was specific to the pollen grain and pollen tube. The expression pattern of these AGPs provides new evidence for the detection of a subset of specific AGPs involved in plant reproductive processes, being of significance for this field of study. AGPs are prominent candidates for male-female communication during reproduction.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLLEN-TUBE GROWTH; EARLY-DIVERGENT ANGIOSPERM; NICOTIANA-ALATA LINK; DOUBLE FERTILIZATION; FLOWERING PLANTS; TRANSMITTING TISSUE; AMARANTHUS-HYPOCHONDRIACUS; TRANSCRIPTIONAL REGULATION; DEVELOPMENTAL REGULATION; PHYSCOMITRELLA-PATENS; Arabinogalactan proteins; female gametophyte; funiculus; pistil; pollen tube guidance; transmitting tract |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Aug 2019 14:03 |
| Last Modified: | 12 Aug 2019 14:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9454 |
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