del Barrio, Melisa and de Marcos, Susana and Cebolla, Vicente and Heiland, Josef and Wilhelm, Stefan and Hirsch, Thomas and Galban, Javier (2014) Enzyme-induced modulation of the emission of upconverting nanoparticles: Towards a new sensing scheme for glucose. BIOSENSORS & BIOELECTRONICS, 59. pp. 14-20. ISSN 0956-5663, 1873-4235
Full text not available from this repository. (Request a copy)Abstract
A new approach for the design of a fluorometric biosensor for continuous monitoring of glucose levels in biological samples based on near-infrared (NIR) excitation is described. The sensor combines the fluorescence of the enzyme glucose oxidase (GOx) chemically modified with a fluorescein derivative (FS) and the luminescent properties of upconverting luminescent nanoparticles (UCLNPs). Both, the chemically modified enzyme (GOx-FS) and the UCLNPs are immobilized in a poly(acrylamide) film as a physical support. The excitation of the UCLNPs with NIR light is of major advantage, since fluorescence background from the matrix is minimized. The upconverted luminescence is used to excite GOx-FS, which undergoes a change in the fluorescence intensity during the enzymatic reaction with glucose. The sensor comprises sufficient stability and covers the physiological range of glucose levels in blood. Furthermore, in a proof of principle experiment, the sensor system responds linearly to glucose concentrations in the range from 33 to 16.6 mM in flow injection analysis mode. (C) 2014 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UP-CONVERSION NANOPARTICLES; LANTHANIDE-DOPED NANOCRYSTALS; NAYF4 NANOCRYSTALS; FLUORESCENCE; OXIDASE; GROWTH; PHASE; Upconverting nanoparticles; Glucose biosensor; Near-infrared excitation; Glucose oxidase |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Aug 2019 08:27 |
| Last Modified: | 14 Aug 2019 08:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9550 |
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