Wilhelm, Stefan and del Barrio, Melisa and Heiland, Josef and Himmelstoss, Sandy F. and Galban, Javier and Wolfbeis, Otto S. and Hirsch, Thomas (2014) Spectrally Matched Upconverting Luminescent Nanoparticles for Monitoring Enzymatic Reactions. ACS APPLIED MATERIALS & INTERFACES, 6 (17). pp. 15427-15433. ISSN 1944-8244,
Full text not available from this repository. (Request a copy)Abstract
We report on upconverting luminescent nanoparticles (UCLNPs) that are spectrally tuned such that their emission matches the absorption bands of the two most important species associated with enzymatic redox reactions. The core-shell UCLNPs consist of a beta-NaYF4 core doped with Yb3+/Tm3+ ions and a shell of pure beta-NaYF4. Upon 980 nm excitation, they display emission bands peaking at 360 and 475 nm, which is a perfect match to the absorption bands of the enzyme cosubstrate NADH and the coenzyme FAD, respectively. By exploiting these spectral overlaps, we have designed fluorescent detection schemes for NADH and FAD that are based on the modulation of the emission intensities of UCLNPs by FAD and NADH via an inner filter effect.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UP-CONVERSION; QUANTUM DOTS; NANOCRYSTALS; NADH; FLUORESCENCE; EMISSION; SHELL; WAVELENGTH; ELECTRODES; THERAPY; upconverting nanoparticles; enzymatic reactions; core-shell nanoparticles; upconversion; inner filter effect |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Aug 2019 09:10 |
| Last Modified: | 14 Aug 2019 09:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9559 |
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