Kopf, Thomas and Schaefer, Hans-Ludwig and Troetzmueller, Martin and Koefeler, Harald and Broenstrup, Mark and Konovalova, Tatiana and Schmitz, Gerd (2014) Influence of Fenofibrate Treatment on Triacylglycerides, Diacylglycerides and Fatty Acids in Fructose Fed Rats. PLOS ONE, 9 (9): e106849. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Fenofibrate (FF) lowers plasma triglycerides via PPAR alpha activation. Here, we analyzed lipidomic changes upon FF treatment of fructose fed rats. Three groups with 6 animals each were defined as control, fructose-fed and fructose-fed/FF treated. Male Wistar Unilever Rats were subjected to 10% fructose-feeding for 20 days. On day 14, fenofibrate treatment (100 mg/kg p.o.) was initiated and maintained for 7 days. Lipid species in serum were analyzed using mass spectrometry (ESI-MS/MS; LCFT-MS, GC-MS) on days 0, 14 and 20 in all three groups. In addition, lipid levels in liver and intestine were determined. Shortchain TAGs increased in serum and liver upon fructose-feeding, while almost all TAG-species decreased under FF treatment. Long-chain unsaturated DAG-levels (36: 1, 36: 2, 36: 4, 38: 3, 38: 4, 38: 5) increased upon FF treatment in rat liver and decreased in rat serum. FAs, especially short-chain FAs (12: 0, 14: 0, 16: 0) increased during fructose-challenge. VLDL secretion increased upon fructose-feeding and together with FA-levels decreased to control levels during FF treatment. Fructose challenge of de novo fatty acid synthesis through fatty acid synthase (FAS) may enhance the release of FAs <= 16: 0 chain length, a process reversed by FF-mediated PPAR alpha-activation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STEAROYL-COA DESATURASE-1; ACTIVATED RECEPTOR-ALPHA; PPAR-ALPHA; GENE-EXPRESSION; OXIDATIVE STRESS; INSULIN-RESISTANCE; METABOLIC SYNDROME; DIABETES-MELLITUS; ENERGY-METABOLISM; LIPID EXTRACTION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Aug 2019 09:56 |
| Last Modified: | 14 Aug 2019 09:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9563 |
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