Eigner, U. and Hiergeist, A. and Veldenzer, A. and Rohlfs, M. and Schwarz, R. and Holfelder, M. (2017) Evaluation of a new real-time PCR assay for the direct detection of diarrheagenic Escherichia coli in stool specimens. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 88 (1). pp. 12-16. ISSN 0732-8893, 1879-0070
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Diarrheagenic E. coli (DEC) are one of the most common causes for diarrhea worldwide, especially in children. We evaluated the rapid RIDA (R) GENE (RG) real-time multiplex PCR assays (R-Biopharm, Darmstadt, Germany) for the detection of the most important diarrheagenic E. coli. Three hundred fifteen liquid or non-formed stool specimens were examined. The results of the RG multiplex assays were compared to specific PCR methods. The sensitivity and specificity of the RG PCRs were as follows, 100%/100% for the detection of EHEC, 963% and 99% for EPEC, 100% and 100% for the detection of EAEC, ETEC and EIEC, respectively. Overall, the RG real-time PCR system for the detection of DEC tested in this study provided reliable and rapid results and shows the ability as a useful addendum for the detection of diarrheagenic E. coli in the medical laboratory. (C) 2017 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MULTIPLEX PCR; INFECTION; PATHOGENS; SAMPLES; PANEL; Diarrheagenic E. coli; RIDA (R) GENE (RG) real-time multiplex PCR assays; Shiga toxin-producing E. coli (STEC); Enteropathogenic E. coil (EPEC); Enteroaggregative E. coli (EAEC); Enterotoxinogenic E. coli (ETEC); Enteroinvasive E. coli (EIEC) |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:10 |
| Last Modified: | 19 Feb 2019 10:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/960 |
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