Liebherr, Raphaela B. and Gorris, Hans H. (2014) Enzyme Molecules in Solitary Confinement. MOLECULES, 19 (9). pp. 14417-14445. ISSN 1420-3049
Full text not available from this repository. (Request a copy)Abstract
Large arrays of homogeneous microwells each defining a femtoliter volume are a versatile platform for monitoring the substrate turnover of many individual enzyme molecules in parallel. The high degree of parallelization enables the analysis of a statistically representative enzyme population. Enclosing individual enzyme molecules in microwells does not require any surface immobilization step and enables the kinetic investigation of enzymes free in solution. This review describes various microwell array formats and explores their applications for the detection and investigation of single enzyme molecules. The development of new fabrication techniques and sensitive detection methods drives the field of single molecule enzymology. Here, we introduce recent progress in single enzyme molecule analysis in microwell arrays and discuss the challenges and opportunities.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLI BETA-GALACTOSIDASE; BACTERIAL-CELL ARRAY; IN-OIL EMULSION; SINGLE-MOLECULE; INDIVIDUAL MOLECULES; FLUORESCENCE SPECTROSCOPY; CAPILLARY-ELECTROPHORESIS; ALKALINE-PHOSPHATASE; DYNAMIC DISORDER; CONFORMATIONAL DYNAMICS; enzyme kinetics; single molecule enzymology; fluorescence microscopy; femtoliter array |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Petra Gürster |
| Date Deposited: | 04 Aug 2020 10:17 |
| Last Modified: | 04 Aug 2020 10:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9614 |
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