Mark, Jonas Josef Peter and Piccinelli, Paolo and Matysik, Frank-Michael (2014) Very fast capillary electrophoresis with electrochemical detection for high-throughput analysis using short, vertically aligned capillaries. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 406 (24). pp. 6069-6073. ISSN 1618-2642, 1618-2650
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A method for conducting fast and efficient capillary electrophoresis (CE) based on short separation capillaries in vertical alignment was developed. The strategy enables for high-throughput analysis from small sample vials (low microliter to nanoliter range). The system consists of a lab-made miniaturized autosampling unit and an amperometric end-column detection (AD) cell. The device enables a throughput of up to 200 separations per hour. CE-AD separations of a dye model system in capillaries of only 4 to 7.5 cm length with inner diameters (ID) of 10 or 15 mu m were carried out under conditions of very high electric field strengths (up to 3.0 kV/cm) with high separation efficiency (half peak widths below 0.2 s) in less than 3.5 s migration time. A non-aqueous background electrolyte, consisting of 10 mM ammonium acetate and 1 M acetic acid in acetonitrile, was used. The practical suitability of the system was evaluated by applying it to the determination of dyes in overhead projector pens.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ZONE-ELECTROPHORESIS; SEPARATION; Fast capillary electrophoresis; Small sample volume; Electrochemical detection; Amperometric detection |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Aug 2019 07:31 |
| Last Modified: | 28 Aug 2019 07:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9645 |
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