Cieplik, Fabian and Spaeth, Andreas and Leibl, Christoph and Gollmer, Anita and Regensburger, Johannes and Tabenski, Laura and Hiller, Karl-Anton and Maisch, Tim and Schmalz, Gottfried (2014) Blue light kills Aggregatibacter actinomycetemcomitans due to its endogenous photosensitizers. CLINICAL ORAL INVESTIGATIONS, 18 (7). pp. 1763-1769. ISSN 1432-6981, 1436-3771
Full text not available from this repository. (Request a copy)Abstract
The aim of this study was to demonstrate that the periodontal pathogen Aggregatibacter actinomycetemcomitans (AA) can be killed by irradiation with blue light derived from a LED light-curing unit due to its endogenous photosensitizers. Planktonic cultures of AA and Escherichia coli were irradiated with blue light from a bluephaseA (R) C8 light-curing unit with an emission peak at 460 nm, which is usually applied for polymerization of dental resins. A CFU-assay was performed for the analysis of viable bacteria after treatment. Moreover, bacterial cells were lysed and the lysed AA and E. coli were investigated for generation of singlet oxygen. Spectroscopic measurements of lysed AA and E. coli were performed and analyzed for characteristic absorption and emission peaks. A light dose of 150 J/cm(2) induced a reduction of a parts per thousand yen5 log(10) steps of viable AA, whereas no effect of blue light was found against E. coli. Spectrally resolved measurements of singlet oxygen luminescence showed clearly that a singlet oxygen signal is generated from lysed AA upon excitation at 460 nm. Spectroscopic measurements of lysed AA exhibited characteristic absorption and emission peaks similar to those of known porphyrins and flavins. AA can be inactivated by irradiation with blue light only, without application of an exogenous photosensitizer. These results encourage further studies on the potential use of these blue light-mediated auto-photosensitization processes in the treatment of periodontitis for the successful inactivation of Aggregatibacter actinomycetemcomitans.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VISIBLE-LIGHT; SINGLET OXYGEN; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; HELICOBACTER-PYLORI; PHOTODYNAMIC INACTIVATION; PORPHYROMONAS-GINGIVALIS; ANTIMICROBIAL RESISTANCE; PROPIONIBACTERIUM-ACNES; FUSOBACTERIUM-NUCLEATUM; STAPHYLOCOCCUS-AUREUS; Photodynamic; Aggregatibacter actinomycetemcomitans; Endogenous photosensitizers; Periodontitis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller Chemistry and Pharmacy > Institut für Organische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Aug 2019 09:18 |
| Last Modified: | 28 Aug 2019 09:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9663 |
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