Managh, Amy J. and Hutchinson, Robert W. and Riquelme, Paloma and Broichhausen, Christiane and Wege, Anja K. and Ritter, Uwe and Ahrens, Norbert and Koehl, Gudrun E. and Walter, Lisa and Florian, Christian and Schlitt, Hans J. and Reid, Helen J. and Geissler, Edward K. and Sharp, Barry L. and Hutchinson, James A. (2014) Laser Ablation-Inductively Coupled Plasma Mass Spectrometry: An Emerging Technology for Detecting Rare Cells in Tissue Sections. JOURNAL OF IMMUNOLOGY, 193 (5). pp. 2600-2608. ISSN 0022-1767, 1550-6606
Full text not available from this repository. (Request a copy)Abstract
Administering immunoregulatory cells to patients as medicinal agents is a potentially revolutionary approach to the treatment of immunologically mediated diseases. Presently, there are no satisfactory, clinically applicable methods of tracking human cells in patients with adequate spatial resolution and target cell specificity over a sufficient period of time. Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) represents a potential solution to the problem of detecting very rare cells in tissues. In this article, this exquisitely sensitive technique is applied to the tracking of gold-labeled human regulatory macrophages (Mregs) in immunodeficient mice. Optimal conditions for labeling Mregs with 50-nm gold particles were investigated by exposing Mregs in culture to variable concentrations of label: Mregs incubated with 3.5 x 10(9) particles/ml for 1 h incorporated an average of 3.39 x 10(8) Au atoms/cell without loss of cell viability. Analysis of single, gold-labeled Mregs by LA-ICP-MS registered an average of 1.9 x 10(5) counts/cell. Under these conditions, 100% labeling efficiency was achieved, and label was retained by Mregs for >= 36 h. Gold-labeled Mregs adhered to glass surfaces; after 24 h of culture, it was possible to colabel these cells with human-specific Sm-154-tagged anti-HLA-DR or Yb-174-tagged anti-CD45 mAbs. Following injection into immunodeficient mice, signals from gold-labeled human Mregs could be detected in mouse lung, liver, and spleen for at least 7 d by solution-based inductively coupled plasma mass spectrometry and LA-ICP-MS. These promising results indicate that LA-ICP-MS tissue imaging has great potential as an analytical technique in immunology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN REGULATORY MACROPHAGES; T-CELLS; TRANSPLANT RECIPIENTS; STEM-CELLS; TRACKING; MICE; IRON; GADOLINIUM; TOLERANCE; CYTOMETRY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Immunologie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Aug 2019 11:37 |
| Last Modified: | 29 Aug 2019 11:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9683 |
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