Wagner, Lysann and Warwick, Rebekah A. and Pannicke, Thomas and Reichenbach, Andreas and Grosche, Antje and Hanani, Menachem (2014) Glutamate release from satellite glial cells of the murine trigeminal ganglion. NEUROSCIENCE LETTERS, 578. pp. 143-147. ISSN 0304-3940, 1872-7972
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It has been proposed that glutamate serves as a mediator between neurons and satellite glial cells (SGCs) in sensory ganglia and that SGCs release glutamate. Using a novel method, we studied glutamate release from SGCs from murine trigeminal ganglia. Sensory neurons with adhering SGCs were enzymatically isolated from wild type and transgenic mice in which vesicular exocytosis was suppressed in glial cells. Extracellular glutamate was detected by microfluorimetry. After loading the cells with a photolabile Ca2+ chelator, the intracellular Ca2+ concentration was raised in SGCs by a UV pulse, which resulted in glutamate release. The amount of released glutamate was decreased in cells with suppressed exocytosis and after pharmacological block of hemichannels. The data demonstrate that SGCs of the trigeminal ganglion release glutamate in a Ca2+-dependent manner. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DORSAL-ROOT GANGLION; SENSORY GANGLIA; RECEPTORS; MECHANISM; NEURONS; COMMUNICATION; HEMICHANNELS; ASTROCYTES; ACTIVATION; EXPRESSION; Gliotransmitter; Glutamate; Satellite glial cell; Trigeminal ganglion; Exocytosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Humangenetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Aug 2019 12:02 |
| Last Modified: | 30 Aug 2019 12:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9728 |
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