Denninger, Philipp and Bleckmann, Andrea and Lausser, Andreas and Vogler, Frank and Ott, Thomas and Ehrhardt, David W. and Frommer, Wolf B. and Sprunck, Stefanie and Dresselhaus, Thomas and Grossmann, Guido (2014) Male-female communication triggers calcium signatures during fertilization in Arabidopsis. NATURE COMMUNICATIONS, 5: 4645. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Cell-cell communication and interaction is critical during fertilization and triggers free cytosolic calcium ([Ca2+](cyto)) as a key signal for egg activation and a polyspermy block in animal oocytes. Fertilization in flowering plants is more complex, involving interaction of a pollen tube with egg adjoining synergid cells, culminating in release of two sperm cells and their fusion with the egg and central cell, respectively. Here, we report the occurrence and role of [Ca2+](cyto) signals during the entire double fertilization process in Arabidopsis. [Ca2+](cyto) oscillations are initiated in synergid cells after physical contact with the pollen tube apex. In egg and central cells, a short [Ca2+](cyto) transient is associated with pollen tube burst and sperm cell arrival. A second extended [Ca2+](cyto) transient solely in the egg cell is correlated with successful fertilization. Thus, each female cell type involved in double fertilization displays a characteristic [Ca2+](cyto) signature differing by timing and behaviour from [Ca2+](cyto) waves reported in mammals.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-VITRO FERTILIZATION; GENETICALLY ENCODED INDICATORS; POLLEN-TUBE RECEPTION; PLASMA-MEMBRANE; EGG ACTIVATION; TROPONIN-C; FLUORESCENT INDICATORS; CYTOPLASMIC CA2+; FLOWERING PLANTS; SYNERGID CELLS; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Sep 2019 10:59 |
| Last Modified: | 04 Sep 2019 10:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9792 |
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