Kesselring, Rebecca and Thiel, Annette and Pries, Ralph and Fichtner-Feigl, Stefan and Brunner, Stefan and Seidel, Philipp and Bruchhage, Karl-Ludwig and Wollenberg, Barbara (2014) The complement receptors CD46, CD55 and CD59 are regulated by the tumour microenvironment of head and neck cancer to facilitate escape of complement attack. EUROPEAN JOURNAL OF CANCER, 50 (12). pp. 2152-2161. ISSN 0959-8049, 1879-0852
Full text not available from this repository. (Request a copy)Abstract
Background: Membrane-bound complement restriction proteins (mCRPs) CD46, CD55 and CD59 enable tumour cells to evade complement dependent cytotoxicity and antibody-dependent killing mechanisms. But less is known about the role of these mCRPs in head and neck cancer. Methods: In this study we determined the expression of the mCRPs on head and neck squamous cell carcinoma (HNSCC) cell lines, on tumour tissue and TDLNs (tumour-draining lymph nodes) as well as on lymphocytes from HNSCC patients. The influence of the HNSCC microenvironment on the mCRP regulation was analysed using Flow Cytometry, Western blotting and small interfering RNAs (siRNA) transfection studies. Results: We examined the effects of the HNSCC tumour milieu on the expression levels of CD46, CD55 and CD59. We investigated the susceptibility of HNSCC cells to CDC (complement-dependent cytotoxicity) while silencing the mCRPs. Our results demonstrate a huge influence of the HNSCC tumour microenvironment on the regulation of mCRP expression and show a reciprocal regulation between the different mCRPs themselves. Conclusions: In summary, our data indicate that HNSCC has evolved different strategies to evade complement attacks and that the tumour microenvironment leads to the enhancement of complement resistance of the surrounding tissue. (C) 2014 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SQUAMOUS-CELL CARCINOMA; RESTRICTION FACTORS CD46; SURFACE EXPRESSION; UP-REGULATION; IMMUNE CELLS; TNF-ALPHA; T-CELLS; INFLAMMATION; MICROBIOTA; CYTOKINES; Complement receptors; CD46; CD55; CD59; Head and neck cancer; HNSCC |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Sep 2019 11:11 |
| Last Modified: | 04 Sep 2019 11:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9801 |
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