Le Thomas, Adrien and Stuwe, Evelyn and Li, Sisi and Du, Jiamu and Marinov, Georgi and Rozhkov, Nikolay and Chen, Yung-Chia Ariel and Luo, Yicheng and Sachidanandam, Ravi and Toth, Katalin Fejes and Patel, Dinshaw and Aravin, Alexei A. (2014) Transgenerationally inherited piRNAs trigger piRNA biogenesis by changing the chromatin of piRNA clusters and inducing precursor processing. GENES & DEVELOPMENT, 28 (15). pp. 1667-1680. ISSN 0890-9369, 1549-5477
Full text not available from this repository. (Request a copy)Abstract
Small noncoding RNAs that associate with Piwi proteins, called piRNAs, serve as guides for repression of diverse transposable elements in germ cells of metazoa. In Drosophila, the genomic regions that give rise to piRNAs, the socalled piRNA clusters, are transcribed to generate long precursor molecules that are processed into mature piRNAs. How genomic regions that give rise to piRNA precursor transcripts are differentiated from the rest of the genome and how these transcripts are specifically channeled into the piRNA biogenesis pathway are not known. We found that transgenerationally inherited piRNAs provide the critical trigger for piRNA production from homologous genomic regions in the next generation by two differentmechanisms. First, inherited piRNAs enhance processing of homologous transcripts into mature piRNAs by initiating the ping-pong cycle in the cytoplasm. Second, inherited piRNAs induce installment of the histone 3 Lys9 trimethylation (H3K9me3) mark on genomic piRNA cluster sequences. The heterochromatin protein 1 (HP1) homolog Rhino binds to the H3K9me3 mark through its chromodomain and is enriched over piRNA clusters. Rhino recruits the piRNA biogenesis factor Cutoff to piRNA clusters and is required for efficient transcription of piRNA precursors. We propose that transgenerationally inherited piRNAs act as an epigenetic memory for identification of substrates for piRNA biogenesis on two levels: by inducing a permissive chromatin environment for piRNA precursor synthesis and by enhancing processing of these precursors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | QUALITY-CONTROL MECHANISM; SMALL RNAS; HETEROCHROMATIN FORMATION; DROSOPHILA-MELANOGASTER; PROTEIN; PIWI; GERMLINE; TRANS; TRANSPOSONS; REPRESSION; H3K9me3; epigenetics; piRNA |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2019 06:49 |
| Last Modified: | 12 Sep 2019 06:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9830 |
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