Penzkofer, A. and Tanwar, M. and Veetil, S. K. and Kateriya, S. and Stierl, M. and Hegemann, P. (2014) Photo-dynamics of BLUF domain containing adenylyl cyclase NgPAC3 from the amoeboflagellate Naegleria gruberi NEG-M strain. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 287. pp. 19-29. ISSN 1010-6030,
Full text not available from this repository. (Request a copy)Abstract
The absorption and emission spectroscopic behavior of the photo-activated adenylyl cyclase NgPAC3 from the amoeboflagellate Naegleria gruberi NEG-M strain was studied. The flavin cofactor was found to be partly fully oxidized and partly fully reduced. The typical BLUF domain (BLUF= (B) under bar lue (L) under baright sensor (U) under bar sing (F) under bar lavin) oxidized flavin absorption photo-cycle dynamics with about 14 nm flavin absorption red-shift in the signaling state was observed. The quantum efficiency of signaling state formation was determined to be phi(s) = 0.66 +/- 0.03. A bi-exponential signaling state recovery to the dark-adapted receptor state was observed with the time constants tau(rec,f) = 275 s and tau(rec,sl) = 45 mm. The thermal irreversible protein unfolding was studied and an apparent protein melting temperature of nu(m) approximate to 50 degrees C was found. The photodynamic behavior of NgPAC3 is compared with the behavior of the previously investigated photo-activated cyclases NgPAC1 (nPAC) and NgPAC2 from the same N. gruberi NEG-M strain. Purified recombinant NgPAC3 showed light-gated adenylate cyclase activity upon illumination with blue light. Its cyclase activity is compared with those of NgPAC1 and NgPAC2. (C) 2014 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MULTIPLE SEQUENCE ALIGNMENT; RHODOBACTER-SPHAEROIDES; FLAVIN MONONUCLEOTIDE; APPA; CELL; PHOTORECEPTOR; CULTIVATION; BIOLOGY; GENOME; Photo-activated cyclase; BLUF domain; Amoeboflagellate Naegleria gruberi; Photo-cycle dynamics; Apparent protein melting temperature; Adenylyl cyclase activity |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2019 07:50 |
| Last Modified: | 12 Sep 2019 07:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9854 |
Actions (login required)
 |
View Item |
