Halbroth, Benedict R. and Heil, Alexander and Distler, Eva and Dass, Martin and Wagner, Eva M. and Plachter, Bodo and Probst, Hans Christian and Strand, Dennis and Hartwig, Udo F. and Karner, Anita and Aichinger, Gerald and Kistner, Otfried and Landfester, Katharina and Herr, Wolfgang (2014) Superior In Vitro Stimulation of Human CD8(+) T-Cells by Whole Virus versus Split Virus Influenza Vaccines. PLOS ONE, 9 (7): e103392. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Pandemic and seasonal influenza viruses cause considerable morbidity and mortality in the general human population. Protection from severe disease may result from vaccines that activate antigen-presenting DC for effective stimulation of influenza-specific memory T cells. Special attention is paid to vaccine-induced CD8(+) T-cell responses, because they are mainly directed against conserved internal influenza proteins thereby presumably mediating cross-protection against circulating seasonal as well as emerging pandemic virus strains. Our study showed that influenza whole virus vaccines of major seasonal A and B strains activated DC more efficiently than those of pandemic swine-origin H1N1 and pandemic-like avian H5N1 strains. In contrast, influenza split virus vaccines had a low ability to activate DC, regardless which strain was investigated. We also observed that whole virus vaccines stimulated virus-specific CD8(+) memory T cells much stronger compared to split virus counterparts, whereas both vaccine formats activated CD4(+) Th cell responses similarly. Moreover, our data showed that whole virus vaccine material is delivered into the cytosolic pathway of DC for effective activation of virus-specific CD8(+) T cells. We conclude that vaccines against seasonal and pandemic (-like) influenza strains that aim to stimulate cross-reacting CD8(+) T cells should include whole virus rather than split virus formulations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENDRITIC CELLS; VACCINATION STRATEGIES; AVIAN INFLUENZA; H5N1 INFLUENZA; A VIRUSES; RESPONSES; IMMUNITY; CULTURE; LYMPHOCYTES; EPITOPES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Sep 2019 13:56 |
| Last Modified: | 30 Sep 2019 13:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9862 |
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