Ziemer, M. and Haeusermann, P. and Janin, A. and Massi, D. and Ziepert, M. and Wolff, D. and Greinix, H. and Hillen, U. (2014) Histopathological diagnosis of graft-versus-host disease of the skin - an interobserver comparison. JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 28 (7). pp. 915-924. ISSN 0926-9959, 1468-3083
Full text not available from this repository. (Request a copy)Abstract
BackgroundHistopathology is an important tool in diagnosing cutaneous graft-versus-host disease (GvHD). Minimum diagnostic criteria for active chronic GvHD have recently been defined. However, they are not specific and their interpretation is dependent on observer judgement. ObjectiveAims of the study: i) to explore interobserver variability in the interpretation of histopathological changes in GvHD, and ii) to analyse the impact of detailed clinical data on histopathological diagnosis of GvHD. MethodsHistopathological slides from 15 skin biopsies of GvHD and from dermatoses with histopathologically similar appearance were sent in two phases to four dermatopathologists experienced in cutaneous GvHD in France, Germany, Italy and Switzerland (first round of blind' review followed by a second round with complete clinical information provided). ResultsInterface dermatitis, especially vacuolar alteration, was the most inconsistently evaluated, particularly in cases with minor alterations. Interestingly, for vacuolar alteration and apoptotic keratinocytes, interobserver variability was lower in the adnexal epithelia than in the interfollicular epidermis. Complete clinical information resulted in increased diagnostic confidence and greater concordance on the final diagnosis, rising from 53% (first round, k=0.345, fair agreement) to 80% (second round, k=0.529, moderate agreement). The percentage of correct diagnoses increased from 33.3% to 80%. ConclusionFor the diagnosis of GvHD, histopathological analysis is of importance, but, for correct diagnosis, the correlation of pathological findings with clinical results is crucial. In cases of minor alteration, histopathologists should focus on the interpretation of vacuolar changes and apoptotic keratinocytes, possibly on the adnexal epithelia.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CLINICAL INFORMATION; HISTORY; ACCURACY; READ; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Oct 2019 13:33 |
| Last Modified: | 23 Jan 2020 08:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9927 |
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