Beck, Hans Christian and Gosau, Martin and Kristensen, Lars Peter and Morsczeck, Christian (2014) A Site-Specific Phosphorylation of the Focal Adhesion Kinase Controls the Formation of Spheroid Cell Clusters. NEUROCHEMICAL RESEARCH, 39 (7). pp. 1199-1205. ISSN 0364-3190, 1573-6903
Full text not available from this repository. (Request a copy)Abstract
Human dental follicle cells (DFCs) are ectomesenchymal multipotent stem cells that form spheroid cell clusters (SCCs) under serum free medium cell culture conditions (SFM). Until today, molecular mechanisms for the formation of SCCs are unknown. In this study a quantitative phosphoproteomics approach revealed regulated phosphorylated proteins in SCCs, which were derived from DFCs after 24 and 48 h in SFM. These regulated proteins were categorized using the Kyoto encyclopedia of genes and genomes program. Here, cellular processes and signaling pathway were identified such as the focal adhesion kinase (FAK) signaling pathway. In addition to the phosphoproteomics approach we showed that a specific phosphorylation of FAK (Y397) was required for the formation of SCCs. In conclusion, this study disclosed the phosphoproteome of SCCs for the first time and showed that the FAK signaling pathway is required for the formation of SCCs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENTAL FOLLICLE PRECURSOR; DIFFERENTIATION IN-VITRO; NEURAL STEM-CELLS; PROGENITOR CELLS; MULLER CELLS; IDENTIFICATION; REPLACEMENT; INHIBITOR; PROTEIN; GROWTH; Dental follicle cells; Phosphoproteomics; Neural differentiation; Focal adhesion kinase |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Oct 2019 09:46 |
| Last Modified: | 17 Oct 2019 09:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9936 |
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