Weiss, Rene and Spittler, Andreas and Schmitz, Gerd and Fischer, Michael B. and Weber, Viktoria (2014) Thrombocyte Adhesion and Release of Extracellular Microvesicles Correlate with Surface Morphology of Adsorbent Polymers for Lipid Apheresis. BIOMACROMOLECULES, 15 (7). pp. 2648-2655. ISSN 1525-7797, 1526-4602
Full text not available from this repository. (Request a copy)Abstract
Whole blood lipid apheresis is clinically applied to reduce low density lipoprotein cholesterol in patients with homozygous familial hypercholesterolemia. Here, we studied the correlation between physicochemical parameters, in particular, surface roughness and blood compatibility, of two polyacrylate-based and a dextran sulfate-based polymer for lipid apheresis. The adsorbent surface roughness was assessed by atomic force microscopy. Freshly isolated human thrombocytes were circulated over adsorbent columns downscaled equivalent to clinical use to study thrombocyte adhesion and microvesicle generation. Quantification of thrombocytes and microvesicles in the flow-through of the columns revealed that both thrombocyte adhesion and microvesicle generation increased with increasing adsorbent surface roughness. Activation of thrombocytes with thrombin receptor-activating peptide-6 favored their adhesion to the adsorbents, as demonstrated by preferential depletion of CD62(+) and PAC-1(+) thrombocytes. Taken together, enhanced polymer surface roughness fostered cell adhesion and microveside release, underscoring the role of extracellular microvesides as markers of cellular activation and of blood compatibility.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LOW-DENSITY-LIPOPROTEIN; PLATELETS; RETENTION; RECEPTORS; PATTERN; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Sep 2019 09:18 |
| Last Modified: | 16 Sep 2019 09:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/9947 |
Actions (login required)
 |
View Item |
